Nisina

Nisin Preparation Specification Sheet

1. Description

NISIN is a natural antibacterial agent based on nisin, which is a polypeptide produced by strains of Lactococcus lactis subsp. lactis.

2. Application Areas

NISIN is effective in a variety of food products across a wide range of pH levels (3.5-6.0), including:

• Processed cheese and cheese spreads, club cheese, blended cheese, direct acidified fresh cheese, and natural ripened cheese.
• Cream products such as flavored, whipped, thickened, and sour cream.
• Dairy and fat-based desserts, yogurt, recombined, and flavored milks.
• Fruit and vegetable preparations, pasteurized fruit juices, plant protein-based desserts, beverages, and coconut milk.
• Dips and snacks.
• Pasteurized liquid egg products.
• Low pH sauces and toppings, including mayonnaise and salad dressings.
• Pasteurized soups and sauces.
• Canned vegetables.
• Processed meats.
• Hot plate flour products, such as crumpets.
• Fermentation processes and fermentation products, such as beer.

3. Potential Benefits

• Effectively inhibits the growth of a wide range of Gram-positive bacteria.
• Extends shelf life.
• Meets consumers’ demand for natural products.
• Enhances product quality.

4. Usage Levels

The recommended dosage of NISIN is in the range of 25-500 mg per kg or liter of food. The exact dosage depends on the nature of the product for which it is intended.

5. Directions for Use

NISIN should be added to heat-processed foods by thoroughly dispersing it in the food substrate prior to the heating process. It can be added directly to the food as a dry powder or as a pre-suspension in water or milk. In certain situations, other methods such as dipping or adding after a fermentation process (e.g., stirred yogurt) may be appropriate. Specialized advice should be sought for these applications.

6. Composition

NISIN is composed of:

• Nisin: Min. 900 IU/mg
• Sodium chloride: Min. 50%
• Water: Max. 3.0%

All percentages are by weight.

7. Physical/Chemical Specifications

Form at 25°C: Powder

Color: White to cream-white

Note: Color variations may occur from batch to batch.

11. Storage Condition

Store unopened between 4-20°C in dry conditions, away from direct sunlight. When opened, store between 4-20°C in the original container, in dry conditions, away from direct sunlight. Shelf life is 24 months when stored according to recommendations.

12. Packaging

NISIN is available in 0.5 kg polyethylene bottles with integral, tamper-proof seals, or according to demand.

13. Purity and Legal Status

Nisin in NISIN is food-grade and meets FAO/WHO, EU, and Food Chemicals Codex specifications and complies with the USA Code of Federal Regulations. Regulations governing the use of Nisin vary between countries. Always consult local food and/or feed regulations concerning the legal status of this product, as legislation may differ by region.

14. Safety and Handling

A Material Safety Data Sheet (MSDS) is available on request.

15. Country of Origin

China.

16. Kosher Status

This product is certified by the Star-K in America.

17. Halal Status

This product is certified Halal by the Majelis Ulama Indonesia.

18. ISO Status

This product is certified by the New Century Inspection and Certification Co., Ltd.

19. HACCP Status

This product is certified by the New Century Inspection and Certification Co., Ltd.

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